Description
SNUPN, also known as KPNBL or RNUT1, is a nuclear import adaptor protein belonging to the Snurportin family. Localizing to the cytoplasm and nucleus, Snurportin-1 contains an N-terminal IBB domain and a trimethylguanosine (m3G)-cap binding domain. It specifically binds the terminal 2,2,7-m3G-cap at the 5' end of U snRNPs and functions to transport U snRNPs into the nucleus via an association with Importin beta. Recombinant human Snurportin-1 protien, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques